Reporter

Part:BBa_K1087020

Designed by: Tong Xu   Group: iGEM13_SCU_China   (2013-09-14)


T7 promoter+constitutive promoter+mRFP1

In this part,the reporter mRFP1 is controlled by both strong T7 promoter and one weak constitutive promoter BBa_J23117. Thus,it's strongly expressed when cotransfected with T7 polymerase while less expressed alone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 61
    Illegal NheI site found at 84
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 670
    Illegal AgeI site found at 782
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None