Part:BBa_K1085045

Pdes-CheY

This biobrick contains the B. subtilis CheY under the promoter of Des (location of promoter see ref. [2]).
CheY is involved in chemotaxis. Phosphorylated CheY interacts with FliM and FliY to cause counterclockwise rotation, which is necessary for the swimming behaviour of the bacteria [1].
The promoter of des is a indirect cold inducible promoter in the desRK system. The temperature switch is at 25°C and 37°C [3].

[1] http://www.uniprot.org/uniprot/P24072
[2] Aguilar, Pablo S., et al. "Molecular basis of thermosensing: a two-component signal transduction thermometer in Bacillus subtilis." The EMBO journal 20.7 (2001): 1681-1691.
[3] Bredeston, L. M., et al. "Thermal regulation of membrane lipid fluidity by a two‐component system in Bacillus subtilis." Biochemistry and Molecular Biology Education 39.5 (2011): 362-366.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 280
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 583
  
• 1000
  COMPATIBLE WITH RFC[1000]