iGEM HokkaidoU Japan 2013 original promoter family is constructed from consensus promoter (BBa_K1084001) by adding mutation at -35 region. Other region is same among family members, and similar with promoter consensus sequence.
Correspondence of sequence and parts number is below.
-35 BBa_ t. e. rank TTGACA K1084001 1 TAGGTC K1084002 2 CTGAAG K1084003 6 GGGGTG K1084004 3 GAGGAT K1084005 5 GCAATA K1084006 7 GGGGGG K1084007 8 TGTGTG K1084008 4 AGTGGG K1084009 9 TCTCGG K1084010 10
t. e. = theoretical transcription efficyency
Promoter transcription efficiency was measured with mRFP1 (BBa_K1084401-K1084410), LacZ and Kanamycine (Km) resistance (BBa_K1084501-K1084505).
Theoretical promoter modeling also has done (see promoter Background page in HokkaidoU 2013 wiki). According to protein, these promoters show relatively another protein activity revel. Choose your best promoter by Promoter Selector. Plate image below is actual worked Promoter Selector (Km resistance).
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC