Generator

Part:BBa_K1083001

Designed by: David Jullesson   Group: iGEM13_Linkoping_Sweden   (2013-09-27)


HEWL-Antigen-Luciferase

This generates a protein construct containing a epitope for HEWL (see biobrick BBa_K1083000). The HEWL epitope (V) is exchangebla due to a built in restriction site for XhoI. H and CH2 are optimezed for E.coli. The VHCH2 generates an antibody from cameloids. This part is connected to Lucifarese (biobrick BBa_K3255209).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1896
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2130
    Illegal SpeI site found at 1896
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1021
    Illegal XhoI site found at 255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1896
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1896
    Illegal NgoMIV site found at 602
    Illegal AgeI site found at 2672
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2186


[edit]
Categories
Parameters
None