Regulatory

Part:BBa_K1082000

Designed by: Gunvor Røkke   Group: iGEM13_NTNU-Trondheim   (2013-09-20)


Pm/xylS promoter system

The Pm/xylS promoter system is positively regulated by m-toluic acid. The m-toluic acid binds to the xylS protein which is constitutively expressed by the Pm/xylS promoter system. The xylS-m-toluic acid complex binds to the Pm promoter, and activates it.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1729
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 930
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1729
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1729
    Illegal NgoMIV site found at 208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 745
    Illegal BsaI site found at 1220


[edit]
Categories
Parameters
None