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Part:BBa_K1081003

Designed by: Tong Liang & Zhaoren He   Group: iGEM13_WHU-China   (2013-09-04)

Tandem Double Promoter->J23102+J23106+B0034 RBS+RFP+B0015 Terminator

This ia a double promoter composed of two consecutive promoters J23102 and J23106. It is added before the target gene to accomplish multiple-stage regulation of transcription. We use RFP reporter to check the initiation efficiency of the double promoter under different conditions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 80
    Illegal PstI site found at 963
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 50
    Illegal NheI site found at 73
    Illegal SpeI site found at 80
    Illegal PstI site found at 963
    Illegal NotI site found at 956
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 80
    Illegal PstI site found at 963
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 80
    Illegal PstI site found at 963
    Illegal AgeI site found at 659
    Illegal AgeI site found at 771
  • 1000
    COMPATIBLE WITH RFC[1000]


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