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Part:BBa_K1076001

Designed by: Luna Lacerda, Adolfo Mota, Carlos Gustavo Nunes   Group: iGEM13_Manaus_Amazonas-Brazil   (2013-09-13)


Flanking genes for FadR deletion


Part of the upstream and dowstream regions of the gene FadR, containing part the genes SpoVR and NhaB respectively.This parts have Nde1 complementary site and they were ligate together. This method garantee generation of a deleted copy of the target gene using a two step assymmetric/crossover PCR amplification. The target in this case is the FadR. In other words its a system to knock down the FadR gene which is transcribed into a regulatory intake fatty-acid protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None