Generator
Part:BBa_K1075046
Designed by: Marc Schulte Group: iGEM13_Bonn (2013-09-23)
pDawn-RBS32-mazF-TT
This part is a combination of the light-inducible BBa_K1075044 and the toxin mazF(BBa_K1075037).
Usage and Biology
pDawn-RBS32-mazF-TT can be used to photocontrol the expression of the toxin mazF. In this system pDawn (BBa_K1075044) can be regulated by blue light, while mazF(BBa_K1075037) acts as a killswitch.
MazF is a toxin, which is counteracted by its antitoxin MazE. MazF exhibits sequence-specific ribonuclease activity toward single- or double-stranded RNA regions, and the resulting degradation of cellular mRNA causes global translation inhibition.[http://www.cell.com/molecular-cell/retrieve/pii/S1097276503004027]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2171
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 63
Illegal NgoMIV site found at 195
Illegal NgoMIV site found at 289
Illegal NgoMIV site found at 582
Illegal NgoMIV site found at 1076
Illegal NgoMIV site found at 1094
Illegal NgoMIV site found at 1184
Illegal AgeI site found at 414
Illegal AgeI site found at 1542 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1643
Illegal BsaI.rc site found at 525
[edit]
Categories
Parameters
None |