Generator
Part:BBa_K1075040
Designed by: Marc Schulte Group: iGEM13_Bonn (2013-09-23)
AraC-pBAD-RBS32-mazF-TT
Structure
This part contains AraC-pBAD(D)-RBS32-mazF-TT
Biology
MazF is part of the biological toxin-antitoxin system MazEF. This system is derived from the bacterium Escherichia coli, where it serves as a stress inducable killswitch. MazF, a RNA-degrading enzyme, ist he toxin and is normally inhibited by the presence of MazE. Absence of MazE leads to cell death. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC532418/]
Application
We used this part to generate a construct of MazF that is inducible by arabinose. This part was used to test the toxicity of MazF as part of our proof of concept.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
[edit]
Categories
Parameters
None |