Generator

Part:BBa_K1075040

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)

AraC-pBAD-RBS32-mazF-TT


Structure

This part contains AraC-pBAD(D)-RBS32-mazF-TT

Biology

MazF is part of the biological toxin-antitoxin system MazEF. This system is derived from the bacterium Escherichia coli, where it serves as a stress inducable killswitch. MazF, a RNA-degrading enzyme, ist he toxin and is normally inhibited by the presence of MazE. Absence of MazE leads to cell death. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC532418/]

Application

We used this part to generate a construct of MazF that is inducible by arabinose. This part was used to test the toxicity of MazF as part of our proof of concept.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None