Generator

Part:BBa_K1075039

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)

pLac-RBS32-mazF-TT

Structure

This part contains pLac1-RBS32-MazF-TT

Biology

MazF is part of the biological toxin-antitoxin system MazEF. This system is derived from the bacterium Escherichia coli, where it serves as a stress inducable killswitch. MazF, a RNA-degrading enzyme, ist he toxin and is normally inhibited by the presence of MazE. Absence of MazE leads to cell death. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC532418/]

Application

We used this part to gain an IPTG and Lactose inducible construct of MazF. This part was used to test the toxicity of MazF as part of our proof of concept.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None