Intermediate

Part:BBa_K1075038

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)

RBS32-MazF-TT

Structure

This part consists of RBS32-MazF()-TT.

Biology

MazF is part of the biological toxin-antitoxin system MazEF. This system is derived from the bacterium Escherichia coli, where it serves as a stress inducable killswitch. MazF, a RNA-degrading enzyme, ist he toxin and is normally inhibited by the presence of MazE. Absence of MazE leads to cell death [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC532418/]. The mutation appeared during cloning processes and it may have an effect on the funcionality of MazF.

Applications

This part can be linked with different promotors to gain an inducible but still inhibitable toxin. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None