Intermediate
Part:BBa_K1075037
Designed by: Marc Schulte Group: iGEM13_Bonn (2013-09-23)
MazF-TT
Structure
This part contains MazF-TT
Biology
MazF is part of the biological toxin-antitoxin system MazEF. This system is derived from the bacterium Escherichia coli, where it serves as a stress inducable killswitch. MazF, a RNA-degrading enzyme, ist he toxin and is normally inhibited by the presence of MazE. Absence of MazE leads to cell death. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC532418/]
Application
This part can be linked with different promotors and ribosomal binding sites to gain an inducible but still inhibitable toxin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |