Intermediate

Part:BBa_K1075037

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)

MazF-TT

Structure

This part contains MazF-TT

Biology

MazF is part of the biological toxin-antitoxin system MazEF. This system is derived from the bacterium Escherichia coli, where it serves as a stress inducable killswitch. MazF, a RNA-degrading enzyme, ist he toxin and is normally inhibited by the presence of MazE. Absence of MazE leads to cell death. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC532418/]

Application

This part can be linked with different promotors and ribosomal binding sites to gain an inducible but still inhibitable toxin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None