Generator

Part:BBa_K1075007

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)

AraC-pBAD2-RBS32-SspB-TT

The sspB protein is an adaptor responsible for delivering ssrA-tagged substrates to the ClpXP protease in order to enhance their degradation. Prefixed is RBS, the binding site for a ribosome and the Arabinose inducable promoter system AraC-pBAd. It also includes a double terminator 'TT', which interrupts the translation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1234
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1069
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1051


[edit]
Categories
Parameters
None