Regulatory

Part:BBa_K1075002

Designed by: Max Schelski   Group: iGEM13_Bonn   (2013-09-22)


Promoter(const.)-AraC-Term-pBAD-RBS32 (Arabinose inducable promoter system)

This is an Arabinose inducable promoter system compromising the repressor AraC and the promoter pBAD as found in the genomic sequence of E. coli. The RBS32 (medium strength) was added downstream of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None