Regulatory

Part:BBa_K1075001

Designed by: Max Schelski   Group: iGEM13_Bonn   (2013-09-22)

Promoter(const.)-AraC-Term-pBAD (Arabinose inducable promoter system)

This part compromises the repressor of the Arabinose operon AraC and the Arabinose inducable promoter pBAD. When AraC is present the promoter pBAD is repressed. Upon induction with Arabinose AraC acts as an activator for the promoter leading to transcription of the downstream gene.

There is no RBS included after the promoter.

It is derived from the expression plasmid pBAD compromising the whole part matching the genomic sequence of this area. Compared to BBa_K808000 there is an additional upstream part of 90bp included.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1234
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1069
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1051


[edit]
Categories
Parameters
None