Regulatory

Part:BBa_K1074012

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-16)


Pgrac promoter

Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG,While the background level of expression of these expression cassettes is very low in the absence of the inducer, an induction factor of about 1,300 was measured using the bgaB reporter gene (Phan et al.,2005). The amount of recombinant protein produced after addition of IPTG may represent 10 and 13%, respectively, of the total cellular protein (demonstrated when fusing the htpG and pbpE genes to the groE promoter; Phan et al., 2005).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None