Composite

Part:BBa_K1074010

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-25)


Pgrac+RBS+SDP

Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of Bacillus subtilis. We put the SDP operon under the strong Regulated promoter Pgrac(BBa_K1074012) to see whether overexpression of spbc can induce the suicide of engineered Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 772
    Illegal PstI site found at 2199
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 772
    Illegal PstI site found at 2199
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 772
    Illegal PstI site found at 2199
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 772
    Illegal PstI site found at 2199
    Illegal AgeI site found at 959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 946


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