Coding

Part:BBa_K1074003

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-09)


Ag85b

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 31
    Illegal PstI site found at 832
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 31
    Illegal PstI site found at 832
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 546
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 31
    Illegal PstI site found at 832
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 31
    Illegal PstI site found at 832
    Illegal NgoMIV site found at 382
    Illegal NgoMIV site found at 436
    Illegal NgoMIV site found at 511
    Illegal NgoMIV site found at 850
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None