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Intermediate

Part:BBa_K1073009

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)

LasI synthetase + LVA with subsequent double terminator

This construct is derived from BBa_C0078 and BBa_B0015. It combines the autoinducer synthase LasI (producing N-3-oxo-dodecanoyl-HSL) including the LVA degradation tag with a double terminator to prevent further transciption.


Usage and Biology

The synthetase LasI produces the autoinducer molecule N-3-oxo-dodecanoyl-HSL of the las quorum sensing system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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