Coding
ampR

Part:BBa_K1073000

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)

ampicillin resistance

This sequence codes for a beta-lactamase, which degrades ampicillin and other beta lactam antibiotics. This brick only consists of the ampR gene and lacks a RBS and promoter. Thus it can be used with an RBS or other device of your choice.

Usage and Biology

The ampicillin resistance gene is used as a selection marker and enables cell growth in the presence of ampicillin due to the degration of ampicillin by the beta-lactamase it codes for.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/selectionmarker/antibioticresistance
Parameters
None