Part:BBa_K1072022

Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+Ndh+2TM

BBa_K1072022 is a composite device, including LuxI,LuxR,GFP and Ndh genes.

LuxR, under the control of Lux pI, generate LuxR protein, which will combined with AHL and activate Lux pR promotor. That complex increases the transcriptional rate of the lux pR promotor while also decrease the transceiptional rate of the Lux pL promoter.

LuxI is the generator of AHL controlled by Lux pR promoter.

Ndh was activated by AHL and LuxR complex and yields H2O2. When H2O2 enters the cell, it transiently changes its redox state and the native aerobic response control systems including ArcAB,which repress Lux pR normally, was inactived.

This part is an improvement of BBa_K546000, made by 2011 iGEM11_Wageningen_UR team. A Lux pR promoter, RBS, Ndh, GFP and double terminator has been added. This part can enhance the array quorum sensing and GFP presents a reproter.

We utilize the characteristic that H2O2 has absorbance at 240nm to complete the linear chart of standard H2O2 concentration as figure 1 by UV spectrophotometer.We choose part BBa_K1072000 as the control for part BBa_K1072022

Figure 1. the linear chart of standard H2O2 concentration

Figure 2. Transforming BBa_K1072022 into E.coli and expressing, we measure H2O2 OD under the control of BBa_K1072000.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1821
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1101
  Illegal BsaI.rc site found at 1970
  Illegal BsaI.rc site found at 2697
  Illegal BsaI.rc site found at 2916