Part:BBa_K1072020

FUS1+BFP+ADH1

In the endogenous MAP-kinase pathway in S.cerevisiae, third messenger,ste12, will stimulate fus1 promoter, which results in mating of the yeast. We create the signal reporter device as “fus1+BFP+ADH1 terminator”. However the sequence of fus1 contains Pst1 restrict enzyme site, we use the method of overlap extension to achieve the purpose of site-directed mutagenesis ("CTGCAG" to "CTGGAG").after transforming Odr-10 and this into yeast, we have detected BFP.

Figure 2. BFP was detected in yeast.

This part is an improvement of BBa_K775004 of 2012 TU delft team;

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]