Composite

Part:BBa_K1072019

Designed by: junjie zhang   Group: iGEM13_SCUT   (2013-09-22)

pGAL1 + Brho + Flag + odr-10 + GFP +ADH1 Te + pFUS1 + BFP + ADH1 Te

This is a GPCR generator of Odr-10 linking Brho signal peptide, Flag tag and reporter gene, BFP.

BBa_K1072015 encodes a diacetyl-sensing G-protein coupled receptor, 7-transmembrane receptors, which can be functionally expressed in S. cerevisiae.

This part is an improvement of BBa_K211002 and BBa_K775003 made by 2009 Hongkong university and 2012 TU delft team, respectively. A Brho signal peptide， flag tag and GFP have been added.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2324


[edit]
Categories
Parameters
None