Composite

Part:BBa_K1072014

Designed by: Junqi kuang, Yaqing du and Xihao liao   Group: iGEM13_SCUT   (2013-09-10)

pGAL1+ Flag + Brho + odr-10 + GFP + ADH1 Te

BBa_K1072014 is protein generator, including Odr-10 and GFP;

BBa_K1072014 encodes a diacetyl-sensing G-protein coupled receptor, 7-transmembrane receptors, which can be functionally expressed in S. cerevisiae. We add Brho signal peptide in C-terminal and link GFP coding region in N-terminal. If cotransformed with BBa_K1072020, Odr10 located at the plasma membrane and activated the downstream MAPK pathway,figure1.

Figure 2. BFP was detected in yeast.



This part is an improvement of BBa_K211002 and BBa_K775003 made by 2009 Hongkong university and 2012 TU delft team, respectively. A Brho signal peptide and GFP have been added.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2324


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Categories
Parameters
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