Part:BBa_K1072007

Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+α-ALS+2TM

BBa_K1072007 is a composite divece, including LuxR, LuxI, GFP and α-ALS genes.

LuxR is under control of a constitutive promotor, Lux pI. Produce, LuxR, forms a complex with the quorum sensing molecule AHL and this complex subsequently increases the transcriptional rate of the lux pR promoter while also decreas the transcriptional rate of the lux pL promoter. The other genes are under control of this lux pR promoter.

The generator, Lux pR+RBS+LuxI+B0015, express AHL which will combine with LuxR.

GFP also is under control of Lux pR, presenting as reporter.

α-ALS，as our desire produce, will transcrip activated by LuxR and AHL complex. If co-transforming with B0015, this system will transcript α-ALS periodically.

Finally, this part just is the combination of BBa_K1072000 and BBa_K1072006.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1821
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1101
  Illegal BsaI.rc site found at 1970
  Illegal BsaI.rc site found at 2697
  Illegal BsaI.rc site found at 2916