Part:BBa_K1072000

Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP

BBa_K1072000 has three protein generators: lux pl-RBS-C0062-B0010-B0012 produces LuxR. LuxR forms a complex with the quorum sensing molecule AHL and this complex subsequently increases the transcriptional rate of the lux pR promoter while also decreas the transcriptional rate of the lux pL promoter. Both of the other generators are under control of this lux pR promoter.

The second generator ‘luxpR-RBS-E0040-B0010-B0012’has a low basal expression of the GFP, which can - of course - be monitored.

The third generator produces luxI, which produces the quorum sensing molecule AHL. In combination with LuxR, AHL thus increases the production of AHL and GFP.

This part is an improvement of BBa_K546000 and BBa_K546005 made by 2011 iGEM11_Wageningen_UR team. A LuxpR promoter, RBS, GFP and double terminator has been added.

Figure 1. GFP was generated, which means LuxR and LuxI expressing successfully

Usage and Biology

Unspecified

Sequence and Feature

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1821
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1101
  Illegal BsaI.rc site found at 1970
  Illegal BsaI.rc site found at 2697