Regulatory

Part:BBa_K1070002

Designed by: Ruosang Qiu   Group: iGEM13_XMU_Software   (2013-09-09)


pBAD with a mutation

For test the influence of sigma factors' strength and the length between them, we made a gene mutation on pBAD, change one of sigma 70 sequence from gatagt into tatagt.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None