Plasmid

Part:BBa_K106701

Designed by: Andrew Horwitz   Group: iGEM08_UCSF   (2008-10-29)


pCR2.1 TOPO Vector (to contain AarI inserts)

This vector was used for TOPO cloning of AarI parts, which could then be sequence verified, and cut out for downstream cloning.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 283
    Illegal EcoRI site found at 301
    Illegal XbaI site found at 346
    Illegal SpeI site found at 258
    Illegal PstI site found at 306
    Illegal PstI site found at 1496
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 283
    Illegal EcoRI site found at 301
    Illegal SpeI site found at 258
    Illegal PstI site found at 306
    Illegal PstI site found at 1496
    Illegal NotI site found at 327
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 283
    Illegal EcoRI site found at 301
    Illegal BglII site found at 1283
    Illegal BamHI site found at 252
    Illegal XhoI site found at 334
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 283
    Illegal EcoRI site found at 301
    Illegal XbaI site found at 346
    Illegal SpeI site found at 258
    Illegal PstI site found at 306
    Illegal PstI site found at 1496
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 283
    Illegal EcoRI site found at 301
    Illegal XbaI site found at 346
    Illegal SpeI site found at 258
    Illegal PstI site found at 306
    Illegal PstI site found at 1496
    Illegal NgoMIV site found at 672
    Illegal NgoMIV site found at 1947
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2846
    Illegal SapI.rc site found at 1796
    Illegal SapI.rc site found at 2006


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Categories
Parameters
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