Part:BBa_K1067004

Hydroxylamine oxidoreductase Hydroxylamine oxidoreductase (HAO) is the second enzyme in the conversion of ammonia to nitrite in Nitrosomonas europaea. It's substrate is hydroxyl amine (NH₂OH) and it's producing nitrite (NO₂^-):

NH₂OH + H₂O + 2 cycAX_ox → NO₂^- + 2 cycAX_red + 5 H⁺

In the brick there is also included a protein putatively involved in the nitrificationhttp://ecocyc.org/NEUR228410/NEW-IMAGE?type=LOCUS-POSITION&object=GJNO-2083&chromosome=CHROMOSOME-1 link to Biocyc.

Usage and Biology

HAO is an integral protein build up on a homotrimeric structure. It's the second step in the enzymatic conversion of ammonia to nitrite in the nitrifying bacteria genus. The protein works in close relationship with cycA, cycX and Ammonium monooxygenase (AMO) in this process. AMO is using 2 free electrons that are made from HAO and picked up by the cytochromes cycA and cycX. For every ammonium molecule 4 electrons are transfer; two of these are redirected to AMO while 2 is picked up and works as energy source for chemolithoautotrophic nitrifying bacteria like Nitrosomonas europaea.

This part is experienced to slow down growth rates of E.coli substantially.

Team Virginia 2017 Part Improvement: https://parts.igem.org/Part:BBa_K2272019

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 811
Illegal PstI site found at 1552
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 811
Illegal PstI site found at 1552
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 811
Illegal BamHI site found at 246
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 811
Illegal PstI site found at 1552
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 811
Illegal PstI site found at 1552
Illegal NgoMIV site found at 2348
Illegal NgoMIV site found at 2382
Illegal AgeI site found at 270
Illegal AgeI site found at 1970
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 328
Illegal BsaI.rc site found at 709