Part:BBa_K1067002

Cytochrome A and X from Nitrosomonas europaea

Cytochrome A and cytochrome X ([http://ecocyc.org/NEUR228410/NEW-IMAGE?type=LOCUS-POSITION&object=GJNO-2382&chromosome=CHROMOSOME-1 link to Biocyc]) both from Nitrosomonas europaea.

The two cytochromes are involved in the process of transferring electrons from HAO to AMO in the following reactions:

HAO reaction, 2 electrons gained:

NH₂OH + H₂O + 2 cycAX_ox → NO₂^- + 2 cycAX_red + 5 H⁺

AMO reaction 2 electrons used:

NH₃ + O₂ + cycAX_red + 2 H⁺ → NH₂OH + H₂O + cycAX_ox

Usage and Biology

When highly expressed in E.coli the cells turn dark red. If grown on media with supplementation of d-aminolevulinic acid (ALA) it tends to get a darker color because of the extra heme incorporated.

https://parts.igem.org/Part:BBa_K2272019 Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 686
  Illegal EcoRI site found at 838
  Illegal PstI site found at 51
  Illegal PstI site found at 490
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 686
  Illegal EcoRI site found at 838
  Illegal PstI site found at 51
  Illegal PstI site found at 490
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 686
  Illegal EcoRI site found at 838
  Illegal BglII site found at 448
  Illegal BamHI site found at 235
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 686
  Illegal EcoRI site found at 838
  Illegal PstI site found at 51
  Illegal PstI site found at 490
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 686
  Illegal EcoRI site found at 838
  Illegal PstI site found at 51
  Illegal PstI site found at 490
  Illegal NgoMIV site found at 1140
  
• 1000
  COMPATIBLE WITH RFC[1000]