Generator
Sir3

Part:BBa_K106695

Designed by: Andrew Horwitz   Group: iGEM08_UCSF   (2008-10-28)


Sir3 under a strong constitutive promoter

Adh1P!-Sir3-Adh1t

Sir3 is expressed from the high constitutive promoter Adh1. This was cloned by AarI, using the A! fusion (no amino acids added to the N-terminus). This is important, as Sir3 does not tolerate N-terminal extensions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2824
    Illegal EcoRI site found at 4422
    Illegal PstI site found at 2263
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2824
    Illegal EcoRI site found at 4422
    Illegal PstI site found at 2263
    Illegal NotI site found at 4470
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2824
    Illegal EcoRI site found at 4422
    Illegal BglII site found at 1832
    Illegal BglII site found at 2205
    Illegal BglII site found at 2427
    Illegal BamHI site found at 4453
    Illegal XhoI site found at 4344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2824
    Illegal EcoRI site found at 4422
    Illegal PstI site found at 2263
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2824
    Illegal EcoRI site found at 4422
    Illegal PstI site found at 2263
    Illegal NgoMIV site found at 229
    Illegal AgeI site found at 2950
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 879
    Illegal BsaI.rc site found at 4161
    Illegal SapI site found at 3061


[edit]
Categories
Parameters
None