Generator

Part:BBa_K106674

Designed by: Jacinto Chen   Group: iGEM08_UCSF   (2008-10-28)


GFP under medium constitutive promoter, 5

8xLexA operator-Cyc1P-GFP-Adh1t

A targeted (LexA Operators) silencing. Contained in the yeast pRS315 integration vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 434
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 434
    Illegal NotI site found at 1169
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1152
    Illegal XhoI site found at 422
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 434
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 434
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1077


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Categories
Parameters
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