Generator

Part:BBa_K106019

Designed by: Andrew Horwitz   Group: iGEM08_UCSF   (2008-10-24)


LexA-Sir2 under a strong constitutive promoter

Adh1P-LexA(1-87)-Sir2-Adh1t

For targeted (LexA Operators) silencing. Contained in the yeast pRS303 integration vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2609
    Illegal BamHI site found at 2047
    Illegal BamHI site found at 2595
    Illegal BamHI site found at 3480
    Illegal XhoI site found at 1505
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 874


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Categories
Parameters
None