Device

Part:BBa_K1058008

Designed by: Tian A'min   Group: iGEM13_BIT   (2013-08-31)

Cr(VI) sensor with egfp(PchrB+chrB+egfp)

This part is composed of the promoter of chromate transporter chrB and the complete coding sequence of chrB. There is an evidence showed that it is sensitive to Cr(VI) and can lead to the expression of downstream egfp.

Usage and Biology

••

iGEM Dundee 2015

This part was ordered from the 2013 submission plates and completely sequenced by iGEM Dundee 2015 and found to be correct. The sequence matched perfectly that of the published sequence from Ochrobactrum tritici 5bvl1. The part was tested as a stand-alone device by iGEM Dundee 2015 and also deconstructed into a new promoter biobrick (BBa_K1590003) and a repressor (chrB) biobrick (BBa_K1590004). Dundee iGEM 2015 found this part to be partially functional but in need of further optimization. More details are located on the 'experience' page.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 135
    Illegal BamHI site found at 1109
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aCr(VI) sensor with egfp(PchrB+chrB+egfp)