Part:BBa_K1057009
pBAD/araC-ferritin_strong
•Over-expression of ferritin didn’t affect the growth so much.
•The cell not expressing overexpression of ferritin, growth inhibition was observed when the concentration of ferrous ascorbate is > 6-8 mM. Virtually, no growth was observed ost cells (both BL21 and SHuffle®) above that concentration.
•The cell expressing human ferritins showed significantly higher tolerance to the ferrous ascorbate.
•We observed significant difference in viability profiles of the two strains BL21 and SHuffle®. In the case of BL21, expression of ferritins improved the cell growth at semi-inhibitory concentration (5-7 mM) of ferrous ascorbate (up to 10 x), but above that concentration, ferritin expression did not rescue the growth of the cell. In case of SHuffle® strain, the final cell density wasn't that elevated, but it rescued the cell growth at higher concentration (7-8 mM) of ferrous ascorbate. We do not know where this difference comes from. It should be noted that SHuffle® is specifically engineered strain so that the cytosolic environment is more oxidizing than the normal E. coli cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1191
Illegal BsaI.rc site found at 2371
Illegal SapI site found at 961
| n/a | pBAD/araC-ferritin_strong |