Composite

Part:BBa_K1053212

Designed by: Yukimi Yamamoto   Group: iGEM13_Tokyo-NoKoGen   (2013-09-11)

Pfixk2- HHR- GFP- DT

Pfixk2 is a promoter activated by phospholylated FixJ response regulator. HHR is Hammerhead Ribozyme and contains GFP's RBS. If HHR does self cleavage, GFP's RBS is exposed, so GFP is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 870
    Illegal XhoI site found at 771
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None