Composite

Part:BBa_K1053207

Designed by: Yukimi Yamamoto   Group: iGEM13_Tokyo-NoKoGen   (2013-09-10)

Pconst.- RBS-YF1/FixJ-DT- Pfixk2- HHR- GFP- DT- Pconst.- taRNA- DT

This part is used negative control against BBa_K1053205. taRNA (taR12) is expressed constantly and YF1 and FixJ is expressed too. In dark YF1 phospholylates FixJ response regulator and FixJ response regulator activates Pfixk2, so HHR is expressed. HHR contains GFP’s RBS. When HHR is expressed, taRNA bind HHR, so HHR doesn’t do self cleavage and GFP’s RBS isn’t exposed, and GFP isn’t expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 108
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 264
    Illegal NheI site found at 287
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3072
    Illegal XhoI site found at 2973
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 108
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 108
    Illegal NgoMIV site found at 862
    Illegal NgoMIV site found at 934
    Illegal NgoMIV site found at 1024
    Illegal NgoMIV site found at 1042
    Illegal NgoMIV site found at 1528
    Illegal NgoMIV site found at 1821
    Illegal NgoMIV site found at 1915
    Illegal AgeI site found at 576
    Illegal AgeI site found at 1696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1585


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