Part:BBa_K1053201

Pconst.-SRⅡ-HtrⅡ-EnvZ-Pompc-HHR*-GFP-DT 　Halophilic archaea, such as Halobacterium salinarum and Natronobacterium pharaonis (N. pharaonis) are known to show phototaxis by the illumination of light by using receptors called sensory rhodopsin I and II (SRI and SRII). The SR proteins are seven-transmembrane retinylidene photoreceptors, which transmits blue light signal (λmax 487 nm) to their corresponding transducers HtrI and HtrII respectively. This two-component system regulates cells’ flagellar motors for phototaxis (Ref. 1, 2). In dark, Sensory rhodopsin phospholylates OmpR response regulator and OmpR response regulator activates P_ompC, so HHR will be expressed. HHR contains GFP’s RBS. When HHR is expressed, HHR cause self cleavage and expose RBS and GFP will express. But this HHR is mutated at active site, and no cleavage activity, so GFP will not be expressed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NotI site found at 1850
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 134
  Illegal NgoMIV site found at 392
  Illegal AgeI site found at 1652
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1088
  Illegal BsaI.rc site found at 1367
  Illegal SapI site found at 862
  Illegal SapI.rc site found at 1868