RNA

Part:BBa_K1053120

Designed by: Yasuhito Ito   Group: iGEM13_Tokyo-NoKoGen   (2013-09-10)


RNA scaffold containing HTLV-1 Rex peptide and λN peptide aptamer

λN peptide aptamer and Rex peptide aptamer are separated by ten consecutive Thymine sequence. When YFP fusion λN peptide and HTLV-1 Rex peptide are co-expressed simultaneously with this aptamer, Split GFP come closer and emit fluorescence by these two peptides bind each aptamer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 74
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None