Coding

Part:BBa_K1053104

Designed by: Eri Takagi   Group: iGEM13_Tokyo-NoKoGen   (2013-09-12)

FB-linker-PP7

Split GFP C-terminal domain (FB) is bound to PP7 through linker.
Split GFP is consisted of two half GFP; FA (N-terminal domain) and FB (C-terminal domain) between amino acid residue 158 and 159. Either FA or FB is not able to emit fluorescence. When FA and FB come closer, the fragments associate and restore fluorescence. Also, PP7 is the single strand RNA phage coat protein which is bound specifically by PP7 aptamer.

Aptamer.GIF


Usage and Biology

Split GFP is used to research RNA detection and localization in vivo by fusion with RNA binding proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 356
    Illegal BglII site found at 612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None