Coding

Part:BBa_K1053103

Designed by: Eri Takagi   Group: iGEM13_Tokyo-NoKoGen   (2013-09-10)

FA-linker-MS2

Split GFP N-terminal domain (FA) is bound to MS2 through linker. Split GFP is consisted of two half GFP; FA (N-terminal domain) and FB (C-terminal domain) between amino acid residue 158 and 159. Either FA or FB is not able to emit fluorescence. When FA and FB come closer, the fragments associate and restore fluorescence. Also, MS2 is the single strand RNA phage coat protein which is bound specifically by MS2 aptamer.

Aptamer.GIF


Usage and Biology

Split GFP is used to research RNA detection and localization in vivo by fusion with RNA binding proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None