Generator

Part:BBa_K1053015

Designed by: Ippei Sakamoto   Group: iGEM13_Tokyo-NoKoGen   (2013-09-27)


PBAD-TR(12)-HHR-taR12y-DT

This hammerhead ribozyme(HHR) has trans-acting RNA(taRNA) responsive region, and this HHR responses to taR12. In absence of taR12, the HHR can self-cleave, but in presence of taR42, their capability of self-cleavage is inhibited due to hybridize with taR12 and change their structures. This HHR has taR12y in the downstream. taR12 and taR12y are orthogonal riboregulators reported by Callura et al.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1338
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1145
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1338
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1338
    Illegal AgeI site found at 980
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 962


[edit]
Categories
Parameters
None