Generator

Part:BBa_K1053014

Designed by: Ippei Sakamoto   Group: iGEM13_Tokyo-NoKoGen   (2013-09-27)


TR(12)-HHR-taR12y

This hammerhead ribozyme(HHR) has trans-acting RNA(taRNA) responsive region, and this HHR responses to taR12. In absence of taR42, the HHR can self-cleave, but in presence of taR12, their capability of self-cleavage is inhibited due to hybridize with taR12 and change their structures. This HHR has taR12y in the downstream. taR12 and taR12y are orthogonal riboregulators reported by Callura et al.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 159
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 159
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 159
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None