Generator

Part:BBa_K1053013

Designed by: Ippei Sakamoto   Group: iGEM13_Tokyo-NoKoGen   (2013-09-27)

PLlacOI-crR12-GFPuv-DT

In riboregulator, a sequence which is complementary to the ribosome binding site (RBS), is inserted upstream of the RBS. After transcription, a stem-loop is formed at the 5’ end of the mRNA, which inhibits ribosome binding and translation (cis-repression). crR12 is described in the reference paper.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 624
    Illegal XhoI site found at 525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None