Generator

Part:BBa_K1053008

Designed by: Ippei Sakamoto   Group: iGEM13_Tokyo-NoKoGen   (2013-09-27)

PBAD-taR12-DT

taRNA(taR12) is transcribed in the presence of arabinose.

using this part, self-cleavage activity of TR(12)-HHR can be controlled.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1264
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1145
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1264
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1264
    Illegal AgeI site found at 980
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 962


[edit]
Categories
Parameters
None