Generator

Part:BBa_K1053005

Designed by: Ippei Sakamoto   Group: iGEM13_Tokyo-NoKoGen   (2013-09-10)

PBAD- TR-HHR*(RBS)-GFPuv-DT


This part consists of a gene that is coding GFPuv and mutant of taRNA responsive HHR which lose self-cleavage activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1856
    Illegal XhoI site found at 1757
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1271
    Illegal SapI site found at 961


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Categories
Parameters
None