Part:BBa_K1051200

Ubiquitin

Purpose

Works for the degradation of fluorescent proteins we used.

Principle

http://upload.wikimedia.org/wikipedia/commons/thumb/5/5b/Ubiquitylation.svg/800px-Ubiquitylation.svg.png
The ubiquitination system.
Ubiquitin,a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).
We want to install ubiquitin to fluorescent proteins at N-terminal.
To test the function of ubiquitin fusion protein, we construct measurement pathway K1051288, which contains J63006, K1051200,K1051003 and K1051006.
Yeast growth curve.
We made the chip as a platform for watching and synchronize the cells. First step is capture the cells by the chip. As showed in the figure, budding yeast can be captured by the chip successfully.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 233
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 25
  Illegal AgeI site found at 253
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Wilkinson KD (October 2005). "The discovery of ubiquitin-dependent proteolysis". Proc. Natl. Acad. Sci. U.S.A. 102 (43): 15280–2. doi:10.1073/pnas.0504842102. PMC 1266097. PMID 16230621.
[2] Kimura Y, Tanaka K (2010). "Regulatory mechanisms involved in the control of ubiquitin homeostasis". J Biochem 147 (6): 793–8. doi:10.1093/jb/mvq044. PMID 20418328.