Generator

Part:BBa_K105017

Designed by: Michael Wild, Katja Karstens   Group: iGEM08_ESBS-Strasbourg   (2008-10-22)


Ura3 - Orotidine-5-phosphate decarboxylase

The Orotidine-5'-phosphate (OMP) decarboxylase catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines in S. cerevisiae. It converts OMP into uridine monophosphate (UMP) and capable to transform 5-FOA into the toxic 5-fluorouracil. Under the name of Ura3 it is one of the commonly used auxotrophic markers in Yeast. There exists a number of S. cerevisiae strains which are deleated for this enzyme. <br\n> <br\n> Our BioBrick contains the protein coding sequence as well as the natural promoter and terminator of the gene. To avoid a transcription of genes downstream of this part, it might be usefull put a strong termintor (i.e. Adh1_term: BBa_J63002) in between the parts.

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 210
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 210
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 210
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 210
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 900
    Illegal SapI.rc site found at 747


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