Generator

Part:BBa_K105016

Designed by: Michael Wild, Katja Karstens   Group: iGEM08_ESBS-Strasbourg   (2008-10-22)

Leu2 - Beta-isopropylmalate dehydrogenase of yeast

The Beta-isopropylmalate dehydrogenase (IMDH) catalyzes the third step in the leucine biosynthesis pathway of S. cerevisiae. Under the name of Leu2 it is one of the commonly used auxotrophic markers in Yeast. There exists a number of S. cerevisiae strains which are deleated for this enzyme.

Our BioBrick contains the protein coding sequence as well as the natural promoter and terminator of the gene. To avoid a transcription of genes downstream of this part, it might be usefull put a strong termintor (i.e. Adh1_term: BBa_J63002) in between the parts.

<br\n> Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1296
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1296
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1296
    Illegal XhoI site found at 2
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1296
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1296
    Illegal AgeI site found at 909
  • 1000
    COMPATIBLE WITH RFC[1000]


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