Generator
Part:BBa_K105016
Designed by: Michael Wild, Katja Karstens Group: iGEM08_ESBS-Strasbourg (2008-10-22)
Leu2 - Beta-isopropylmalate dehydrogenase of yeast
The Beta-isopropylmalate dehydrogenase (IMDH) catalyzes the third step in the leucine biosynthesis pathway of S. cerevisiae. Under the name of Leu2 it is one of the commonly used auxotrophic markers in Yeast. There exists a number of S. cerevisiae strains which are deleated for this enzyme.
Our BioBrick contains the protein coding sequence as well as the natural promoter and terminator of the gene. To avoid a transcription of genes downstream of this part, it might be usefull put a strong termintor (i.e. Adh1_term: BBa_J63002) in between the parts.
<br\n> Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1296
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1296
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1296
Illegal XhoI site found at 2 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1296
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1296
Illegal AgeI site found at 909 - 1000COMPATIBLE WITH RFC[1000]
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Parameters
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