Plasmid

Part:BBa_K105000

Designed by: Manuel Gersbacher, Robin Sorg, Marius Müller, Paul Bourgine, Sandra Dittmann, Michi Wild und Katja Karstens   Group: iGEM08_ESBS-Strasbourg   (2008-10-28)


pSB1A2_fusion

This BioBrick correspond to the (pSB1A2) vector. Only the first and the last base have been removed, so this vector is appropriated for the construction of fusion proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2057
    Illegal XbaI site found at 2072
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2057
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
    Illegal NotI site found at 2063
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2057
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 2057
    Illegal XbaI site found at 2072
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2057
    Illegal XbaI site found at 2072
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1096


[edit]
Categories
Parameters
None